Directed evolution of novel polymerase activities: mutation of a DNA polymerase into an efficient RNA polymerase.

作者: G. Xia , L. Chen , T. Sera , M. Fa , P. G. Schultz

DOI: 10.1073/PNAS.102577799

关键词:

摘要: … evolve DNA polymerases with RNA polymerase activity. The Stoffel fragment (SF) of Thermus aquaticus DNA polymerase … Phage particles displaying SF polymerases, which are able to …

参考文章(28)
Clyde A. Hutchison, Ronald Swanstrom, Daniel D. Loeb, Complete mutagenesis of protein coding domains. Methods in Enzymology. ,vol. 202, pp. 356- 390 ,(1991) , 10.1016/0076-6879(91)02019-6
L. Rowen, A. Kornberg, A ribo-deoxyribonucleotide primer synthesized by primase. Journal of Biological Chemistry. ,vol. 253, pp. 770- 774 ,(1978) , 10.1016/S0021-9258(17)38169-3
Jean-Luc Jestin, Peter Kristensen, Greg Winter, A Method for the Selection of Catalytic Activity Using Phage Display and Proximity Coupling Angewandte Chemie International Edition. ,vol. 38, pp. 1124- 1127 ,(1999) , 10.1002/(SICI)1521-3773(19990419)38:8<1124::AID-ANIE1124>3.0.CO;2-W
Eunju Lee Tae, Yiqin Wu, Gang Xia, Peter G. Schultz, Floyd E. Romesberg, Efforts toward expansion of the genetic alphabet: replication of DNA with three base pairs. Journal of the American Chemical Society. ,vol. 123, pp. 7439- 7440 ,(2001) , 10.1021/JA010731E
Huimin Zhao, Lori Giver, Zhixin Shao, Joseph A. Affholter, Frances H. Arnold, Molecular evolution by staggered extension process (StEP) in vitro recombination Nature Biotechnology. ,vol. 16, pp. 258- 261 ,(1998) , 10.1038/NBT0398-258
Edward Arnold, Jianping Ding, Stephen H. Hughes, Zdenek Hostomsky, Structures of DNA and RNA polymerases and their interactions with nucleic acid substrates Current Opinion in Structural Biology. ,vol. 5, pp. 27- 38 ,(1995) , 10.1016/0959-440X(95)80006-M
Rui Sousa, Structural and mechanistic relationships between nucleic acid polymerases Trends in Biochemical Sciences. ,vol. 21, pp. 186- 190 ,(1996) , 10.1016/S0968-0004(96)10023-2
Arnold R Oliphant, Alexander L. Nussbaum, Kevin Struhl, Cloning of random-sequence oligodeoxynucleotides. Gene. ,vol. 44, pp. 177- 183 ,(1986) , 10.1016/0378-1119(86)90180-0
G. A. Rice, C. M. Kane, M. J. Chamberlin, Footprinting analysis of mammalian RNA polymerase II along its transcript: an alternative view of transcription elongation. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 88, pp. 4245- 4249 ,(1991) , 10.1073/PNAS.88.10.4245