Functional Tethered Bilayer Lipid Membranes

摘要: Tethered bilayer lipid membranes (tBLMs) constitute a novel experimental concept with very promising features for fundamental biophysical investigations of the general correlation between structural properties and functional processes in model membrane systems. Moreover, these architectures offer robust platform membrane-based bio-sensing principles that could eventually result design fabrication stable cheap chips. We first discuss few synthetic strategies lead to various types unprecedented electrical properties: some tBLMs show an resistance exceeds even best known so far, i.e., bimolecular (BLM). The reconstitution ionophore, carrier valinomycin, allows one study K+-selective reversible increase conductivity by more than 4 orders magnitude. Next, incorporation integrin receptors into tethered will be briefly discussed as example versatility this system binding assays. And finally, photopolymerization polymerizable lipids introduced way generate patterned bilayers can serve basic structure construction chips massive paralleled detection processes.