Incorporation of the acetylcholine receptor dimer from Torpedo californica in a peptide supported lipid membrane investigated by surface plasmon and fluorescence spectroscopy
作者： E.K. Schmidt , T. Liebermann , M. Kreiter , A. Jonczyk , R. Naumann
DOI: 10.1016/S0956-5663(98)00013-X
关键词:
摘要: Abstract The dimer species (Mr 580 000) of the nicotinic acetylcholine receptor, isolated from electric organ Torpedo californica, was incorporated into a thiopeptide supported lipid bilayer. incorporation achieved by fusion liposomes with reconstituted receptor onto gold-supported monolayer. Surface plasmon resonance spectroscopy (SPS) used to monitor in real time process as well specific binding antagonist α-bungarotoxin. A recently developed extension SPS offering enhanced sensitivity and specificity, surface fluorescence (SPFS), then subsequent monoclonal WF6 polyclonal antibody, respectively. latter labeled Cy5. different assays indicate successful
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