作者:
关键词: LNCaP 、 Cancer research 、 Cell migration 、 Carboxylesterase 1 、 Apoptosis 、 Pharmacology 、 Protein methylation 、 Cancer 、 Biology 、 Prostate cancer 、 Viability assay
摘要: Prostate cancer (CaP) is the most frequently diagnosed in US men, with an estimated 236,590 new cases and 29,720 deaths 2013. There exists need to identify biomarkers/therapeutic targets for early/companion diagnosis development of novel therapies against recalcitrant disease. Mutation overexpression-induced abnormal activities polyisoprenylated proteins have been implicated CaP. Polyisoprenylated methylated protein methyl esterase (PMPMEase) catalyses only reversible terminal reaction polyisoprenylation pathway may promote effects G on cell viability. In this review, potential role PMPMEase serve as a drug target androgen-insensitive CaP was determined. Specific were found be 3.5- 4.5-fold higher androgen-sensitive 22Rv1 androgen-dependent LNCaP 1.5- 9.8-fold castration-resistant DU 145 PC-3 cells compared normal WPE1-NA22 prostate cells. The inhibitor, L-28, induced apoptosis EC50 values ranging from 1.8 4.6 μM. activity following treatment L-28 followed similar profile, IC50 2.3 130 disrupted F-actin filament organisation at 5 μM inhibited migration 4-fold 2 Analysis tissue microarray expression revealed intermediate, strong, very strong staining 94.5% 92 adenocarcinoma trace weak normal-adjacent controls. data are indication that effective targeting through more potent agents lead successful metastatic