Chapter 9 Reconstitution of Epithelial Ion Channels
作者： Robert J. Bridges , Dale J. Benos
DOI: 10.1016/S0070-2161(08)60235-X
关键词: Ion channel 、 Chemistry 、 Biochemistry 、 Direct transfer 、 Biophysics 、 Synthetic membrane 、 Bilayer 、 Planar lipid bilayer
摘要: Publisher Summary This chapter focuses on the reconstitution of ion channels, emphasizing both functional and biochemical approaches. Functional means direct transfer an channel from its native membrane into a planar lipid bilayer, whereas refers to detergent extraction purification subsequent reinsertion back defined environment. The summarizes experimental approaches used in isolation channels discusses bilayer techniques studies. It also provides description incorporation process advantages disadvantages each method using specific examples studied fashion. considers studies Na + , CI – K epithelial tissues. concludes by giving projections expectations future field.
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