Preparation of an Orthotopic, Syngeneic Model of Lung Adenocarcinoma and the Testing of the Antitumor Efficacy of Poly(2-oxazoline) Formulation of Chemo-and Immunotherapeutic Agents

摘要: Tumor xenograft models developed by transplanting human tissues or cells into immune-deficient mice are widely used to study cancer response drug candidates. However, unfit for investigating the effect of immunotherapeutic agents on host immune (Morgan, 2012). Here, we describe preparation an orthotopic, syngeneic model lung adenocarcinoma (LUAD), a subtype non-small cell (NSCLC), antitumor chemo and in immune-competent animal. The tumor is implanting 344SQ LUAD derived from metastases KrasG12D; p53R172HΔG genetically engineered mouse left (Sv/129). offers several advantages over other models: 1) allows assessment biologic effects immune-modulating agents; 2) pathophysiological features disease preserved due orthotopic approach; 3) Predisposition metastasize facilitates therapeutic primary as well ( Chen et al., 2014 ). Furthermore, also treatment strategy based Poly(2-oxazoline) micelles that has been shown be effective this difficult-to-treat Vinod 2020b