Epigenetic status of buffalo fibroblasts treated with sodium butyrate a chromatin remodeling agent.
作者： Papori Sharma , A.S. Yadav , N.L. Selokar , Dharmendra Kumar , S.S. Dhaka
DOI: 10.1016/J.TICE.2017.12.006
关键词: Epigenetics 、 DNA methylation 、 Cell culture 、 Sodium butyrate 、 Chromatin remodeling 、 Reprogramming 、 Fibroblast 、 Somatic cell nuclear transfer 、 Biology 、 Cell biology
摘要: Abstract The somatic cells having higher levels of DNA methylation and reducing it in donor before nuclear transfer (NT) by treating them with chemicals, may improve cloning efficiency NT embryos providing similar epigenetic characteristics as vivo embryos. Therefore, the present study was planned to understand mechanism changes (buffalo fibroblasts) treated different concentration sodium butyrate (NaBu)-a chromatin remodeling agent. cultured fibroblasts purity lineage were confirmed fibroblast specific protein gene markers (Vimentin, Tubulin Cytokeratin) at passages using immuno-staining qPCR respectively. buffalo 1, 3 5 mM NaBu observations taken on their morphological changes, population doubling time cell proliferation after 48 h treatment. observed acetylation (H3K9ac) (H3K27me3) expression. derived from new born ear tissue started emerging anchoring culture flasks within 24 h showed spindle-shaped morphology. confluent cryopreserved interval. post thaw behavior also interval passages. morphology changed increase dosages times decreases rate dose dependent manner. treatment that significantly increased level over control. Based characterization well modifications these NaBu, results suggest provide a useful approach for better reprogramming SCNT
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