Proteomic Analysis of Anticancer TCMs Targeted at Mitochondria

摘要: Traditional Chinese medicine (TCM) is a rich resource of anticancer drugs. Increasing bioactive natural compounds extracted from TCMs are known to exert significant antitumor effects, but the action mechanisms far clear. Proteomics, powerful platform comprehensively profile drug-regulated proteins, has been widely applied mechanistic investigation and identification drug targets. In this paper, we discuss several TCM products including terpenoids, flavonoids, glycosides that were extensively investigated by proteomics illustrate their in various cancers. Interestingly, many these isolated mostly tumor-suppressing functions specifically targeting mitochondria cancer cells. These components induce loss mitochondrial membrane potential, release cytochrome c, accumulation ROS, initiating apoptosis cascade signaling. Proteomics provides systematic views help understand molecular tumor cells; it bears inherent limitations uncovering drug-protein interactions, however. Subcellular fractionation may be coupled with capture identify target proteins mitochondria-enriched lysates. Furthermore, translating mRNA analysis, new technology profiling genes translatome level, integrated into investigation, revealing global information valuable for understanding mechanism TCMs.