Fibrous Proteins of Bovine Hoof

摘要: The matrix region of calf hoof was identified as the living precursor layer hardened plate. Fibrous protein isolated from with citrate buffer, pH. 2.65, while Tris 9.5, 8 M urea and a reducing agent required to dissolve cornified hoof. purified had sodium dodecyl sulfate-polyacrylamide gel electrophoretic pattern similar plate different bovine epidermis. When s-carboxymethyl derivatives matrix, plate, hair proteins compared by urea-polyacrylamide electrophoresis, they were identical, that epidermal protein. reacted an antibody fibrous Cultured keratinocytes appeared be identical cultured cells, pointing importance dermis in cell differentiation.