Striatal neurons in striatal grafts are derived from both post-mitotic cells and dividing progenitors.

摘要: Transplants of embryonic striatal tissue are characteristically heterogeneous, containing patches (P-zones) medium spiny projection neurons. It is not yet known how this morphology develops, and whether the neurons in grafts derived from post-mitotic neuroblasts brain or progenitors that continue to divide after transplantation. To address question we labelled dividing cells transplants with bromodeoxyuridine (BrdU), either prior transplantation into adult lesioned rat striatum. Cells for were pre-labelled utero by intraperitoneal (i.p.) injections BrdU, post-labelled i.p. hosts. Either two six months brains processed using double immunohistochemical techniques detect BrdU calbindin-positive transplants. In approximately 30% heavily suggesting these had undergone a final division where been post-transplantation, 17% calbindin labelled. These results suggest whereas proportion at time transplantation, other progenitor can then complete an appropriate neuronal maturation programme host environment.