Peroxiredoxin 6 as an antioxidant enzyme: protection of lung alveolar epithelial type II cells from H2O2-induced oxidative stress.

摘要: We evaluated the antioxidant role of peroxiredoxin 6 (Prdx6) in primary lung alveolar epithelial type II cells (AEC II) that were isolated from wild (WT), Prdx6−/−, or Prdx6 transgenic (Tg) overexpressing mice and exposed to H2O2 at 50–500 µM for 1–24 h. Expression Tg AEC was sevenfold greater than WT. null showed concentration-dependent cytotoxicity indicated by decreased “live/dead” cell ratio, increased propidium iodide (PI) staining, annexin V binding, DNA fragmentation TUNEL assay, lipid peroxidation diphenylpyrenylphosphine (DPPP) fluorescence. Compared cells, oxidant-mediated damage significantly less WT least cells. Thus, functions as an enzyme mouse II. has been shown previously reduce phospholipid hydroperoxides we postulate this activity is a major mechanism effectiveness enzyme. J. Cell. Biochem. 104: 1274–1285, 2008. © 2008 Wiley-Liss, Inc.