Immuno-MS based targeted proteomics: highly specific, sensitive, and reproducible human chorionic gonadotropin determination for clinical diagnostics and doping analysis.
作者： Hanne Lund , Karoline Løvsletten , Elisabeth Paus , Trine Grønhaug Halvorsen , Léon Reubsaet
DOI: 10.1021/AC301418F
关键词: Biomarker (cell) 、 Analytical chemistry 、 Selected reaction monitoring 、 Targeted proteomics 、 Chromatography 、 Human chorionic gonadotropin 、 Chemistry 、 Serum samples 、 Monoclonal antibody 、 Detection limit 、 Bioanalysis
摘要: The human chorionic gonadotropin (hCG) proteins constitute a diverse group of molecules that displays biomarker value in pregnancy detection and cancer diagnostics, as well doping analysis. For the quantification hCGβ qualitative differentiation between other hCG variants selective, sensitive, reproducible manner, targeted proteomics approach based on mass spectrometric (MS) selected reaction monitoring (SRM) was exploited. By optimizing immunoaffinity extraction using monoclonal antibodies coated to magnetic beads, access granted for MS low-abundance target proteins, ensuring proper sensitivity with limits (LODs) 2 5 IU/L, respectively, urine serum samples. Validation according key elements recommendations defined by European Medicines Agency Guideline Bioanalytical Methods performed. both matrixes this demonstrated good within-day precision results (within 20% lowest concentratio...
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