Middle region of FancM interacts with Mhf and Rmi1 in silkworms, a species lacking the Fanconi anaemia (FA) core complex
作者： R. Sugahara , H. Mon , J. M. Lee , T. Kusakabe
DOI: 10.1111/IMB.12072
关键词: FANCM 、 FANCA 、 DNA 、 DNA repair 、 Genetics 、 Biology 、 Protein subunit 、 FANCD2 、 Monoubiquitination 、 RMI1
摘要: The Fanconi anaemia (FA) pathway is responsible for interstrand crosslink (ICL) repair. Among the FA core complex components, FANCM believed to act as a damage sensor ICL-blocked replication fork and also molecular platform assembly interaction with Bloom's syndrome (BS) that thought play an important role in processing of DNA structures such stalled forks. In present study, we found silkworms, Bombyx mori, species lacking major components (FANCA, B, C, E, F, G), FancM required FancD2 monoubiquitination cell proliferation presence mitomycin C (MMC). Silkworm (BmFancM) was phosphorylated middle regions, modification associated its subcellular localization. addition, BmFancM interacted Mhf1, histone-fold protein, Rmi1, subunit BS complex, different regions. region containing at least these two protein-binding domains played essential FancM-dependent resistance MMC. Our results suggest acts recruitment both protein although silkworm genome seems lose FAAP24, FancM-binding partner mammals.
elsevier.com
sci-hub.se 参考文章(32)
Tsuneyuki Tatsuke, Yutaka Kawaguchi, Jae Man Lee, Takahiro Kusakabe, Sun Mee Hong, Hiroaki Mon, Hiromi Tobata, Construction of piggyBac-based vectors using visible and drug-resistance marker for introducing foreign genes into silkworm cultured cells Journal of The Faculty of Agriculture Kyushu University. ,vol. 54, pp. 397- 400 ,(2009) , 10.5109/16121
Hitoshi Mitsunobu, Yutaka Kawaguchi, Jae Man Lee, Takahiro Kusakabe, Hiroaki Mon, Katsumi Koga, Kosuke Sakashita, Hiroko Yoshida, Construction of gateway-based destination vectors for detecting subcellular localization of proteins in the silkworm, Bombyx mori Journal of insect biotechnology and sericology. ,vol. 75, pp. 141- 145 ,(2006) , 10.11416/JIBS.75.141
Thiyam Ramsing Singh, Dorina Saro, Abdullah Mahmood Ali, Xiao-Feng Zheng, Chang-hu Du, Michael W. Killen, Aristidis Sachpatzidis, Kebola Wahengbam, Andrew J. Pierce, Yong Xiong, Patrick Sung, Amom Ruhikanta Meetei, MHF1-MHF2, a Histone-Fold-Containing Protein Complex, Participates in the Fanconi Anemia Pathway via FANCM Molecular Cell. ,vol. 37, pp. 879- 886 ,(2010) , 10.1016/J.MOLCEL.2010.01.036
Miho Amano, Aussie Suzuki, Tetsuya Hori, Chelsea Backer, Katsuya Okawa, Iain M. Cheeseman, Tatsuo Fukagawa, The CENP-S complex is essential for the stable assembly of outer kinetochore structure Journal of Cell Biology. ,vol. 186, pp. 173- 182 ,(2009) , 10.1083/JCB.200903100
Larry H. Thompson, Nigel J. Jones, Stabilizing and remodeling the blocked DNA replication fork: anchoring FANCM and the Fanconi anemia damage response. Molecular Cell. ,vol. 37, pp. 749- 751 ,(2010) , 10.1016/J.MOLCEL.2010.03.003
Irene Garcia-Higuera, Toshiyasu Taniguchi, Shridar Ganesan, M.Stephen Meyn, Cynthia Timmers, James Hejna, Markus Grompe, Alan D D'Andrea, Interaction of the Fanconi anemia proteins and BRCA1 in a common pathway. Molecular Cell. ,vol. 7, pp. 249- 262 ,(2001) , 10.1016/S1097-2765(01)00173-3
Hiroaki Mon, Ryohei Sugahara, Sun-Mee Hong, Jae-Man Lee, Yusuke Kamachi, Yutaka Kawaguchi, Takahiro Kusakabe, Analysis of protein interactions with two-hybrid system in cultured insect cells. Analytical Biochemistry. ,vol. 392, pp. 180- 182 ,(2009) , 10.1016/J.AB.2009.05.033
Kenta Nakai, Paul Horton, PSORT: a program for detecting sorting signals in proteins and predicting their subcellular localization. Trends in Biochemical Sciences. ,vol. 24, pp. 34- 35 ,(1999) , 10.1016/S0968-0004(98)01336-X
Thiyam Ramsing Singh, Sietske T. Bakker, Sheba Agarwal, Michael Jansen, Elke Grassman, Barbara C. Godthelp, Abdullah Mahmood Ali, Chang-hu Du, Martin A. Rooimans, Qiang Fan, Kebola Wahengbam, Jurgen Steltenpool, Paul R. Andreassen, David A. Williams, Hans Joenje, Johan P. de Winter, Amom Ruhikanta Meetei, Impaired FANCD2 monoubiquitination and hypersensitivity to camptothecin uniquely characterize Fanconi anemia complementation group M Blood. ,vol. 114, pp. 174- 180 ,(2009) , 10.1182/BLOOD-2009-02-207811
Feng Wang, Yuting Yang, Thiyam Ramsing Singh, Valeria Busygina, Rong Guo, Ke Wan, Weidong Wang, Patrick Sung, Amom Ruhikanta Meetei, Ming Lei, Crystal Structures of RMI1 and RMI2, Two OB-Fold Regulatory Subunits of the BLM Complex Structure. ,vol. 18, pp. 1159- 1170 ,(2010) , 10.1016/J.STR.2010.06.008