A specific prebiotic oligosaccharide mixture stimulates delayed-type hypersensitivity in a murine influenza vaccination model.

摘要: Analogous to reported immunomodulatory effects of probiotics, this study was performed analyse the properties prebiotic oligosaccharides that share chemical characteristics with human milk oligosaccharides. A mixture containing galacto- and fructo-oligosaccharides (GOS/FOS; ratio 9:1) tested at dietary doses between 1% 10% (w/w total diet) in an influenza vaccination model, using 10 C56BL/6JolaHsd mice per group. The modulation vaccine specific delayed-type hypersensitivity (DTH) responses studied as a marker T-helper 1 (Th1) immunity, well other immune parameters. GOS/FOS enhanced DTH dose-dependently (optimum 5% w/w diet; 41.4+/-14.1% increased compared controls, p<0.05). No significant changes were detected on splenocyte proliferation or vaccine-specific antibody concentrations. Simultaneously, proportion faecal bifidobacteria lactobacilli (maximal effect 16.8+/-2.4% 5.8+/-1.3% controls respectively, p<0.01 for both parameters). In comparative experiment, FOS/inulin (both 2% induced similar gut microbiota. contrast GOS/FOS, did not enhance responses, indicating increase proportions is sufficient model. use products might provide opportunity stimulate adaptive response Th1-direction subsequently inhibit infections Th2-related disorders humans, instance allergies. Clinical studies are being confirm this.