In vivo protein cross-linking.

摘要: In the cell, homo- and heteroassociations of polypeptide chains evolve take place within subcellular compartments that are crowded with many other cellular macromolecules. vivo chemical cross-linking proteins is a powerful method to examine changes in protein oligomerization protein-protein interactions upon events such as signal transduction. This chapter intended provide guide selection cell-membrane-permeable cross-linkers, optimization conditions, identification specific cross-links context where frequency random collisions high. By combining chemoselectivity homo-bifunctional cross-linker length its spacer arm knowledge on structure, we show selective can be introduced specifically either dimer or hexamer form same vitro well vivo, using human type B nucleoside diphosphate kinase model.