作者: Bjoern Liaset , E Lied , M Espe
DOI: 10.1002/(SICI)1097-0010(200004)80:5<581::AID-JSFA578>3.0.CO;2-I
关键词: Enzyme 、 Chemistry 、 Protein digestion 、 Enzymatic hydrolysis 、 Hydrolysis 、 Proteolysis 、 Atlantic cod 、 Chromatography 、 Food science 、 Hydrolysate 、 Pepsin
摘要: Fish frames without heads from Atlantic cod and salmon were proteolysed with the industrial enzymes neutrase®, alcalase® pepsin for 1, 15, 30, 45, 60, 90 120 min. After 120 min of hydrolysis, treated alcalase yielded significantly (p < 0.05) higher protein recoveries (67.6 64% respectively) as compared to neutrase or (53–62%). To minimise bitterness in fish hydrolysates, kojizyme™ was added after pre-hydrolysis alcalase, hydrolysis run additional times 120, 240, 360, 480, 600 720 min. Protein recovery did not change during kojizyme, but degree increased (p < 0.01) both hydrolysates. A hydrolysate (150 min) followed by treatment kojizyme (510 min) produced. The final freeze-dried a (FPH) chemically characterised. nutritional value FPH established an experiment rats. Inclusion 10% FPH-N showed rats fed inclusion levels FPH. © 2000 Society Chemical Industry