作者: Muhammad Ayaz , Rafiq Ahmad , Muhammad Shahzad , Noorullah Khan , Mohammad Maroof Shah
DOI: 10.1016/J.SCIENTA.2015.08.025
关键词: Biomass 、 Shoot 、 Abiotic stress 、 Crop 、 Germination 、 Drought tolerance 、 Phenotypic trait 、 Transformation (genetics) 、 Biology 、 Agronomy
摘要: Abstract Tomato is an important agricultural crop and facing severe drought stress. PEG-6000 in five different concentrations was used to artificially induced stress two tomato varieties; Nagina 17905.The results demonstrated that seed germination significantly reduced upto 60% for variety at 8% PEG-6000. Other phenotypic traits such as shoot length, leaf leaves number area also with increasing concentration of PEG-6000, however, root length increased concentration. Fresh biomass both varieties found maximum 1.51 g 2.50 g 2% minimum 1.20 g 1.14 g respectively. However, showed more tolerance than 17,905 concentrations. At molecular level, the three selected genes i.e. SlAREB, SlNCED3, SlERF024 were be over expressed In silico characterization these revealed conserved protein domains, involved These concurred conclude plant could produce tolerant through transformation.