Drought stress stunt tomato plant growth and up-regulate expression of SlAREB, SlNCED3, and SlERF024 genes

作者: Muhammad Ayaz , Rafiq Ahmad , Muhammad Shahzad , Noorullah Khan , Mohammad Maroof Shah

DOI: 10.1016/J.SCIENTA.2015.08.025

关键词: BiomassShootAbiotic stressCropGerminationDrought tolerancePhenotypic traitTransformation (genetics)BiologyAgronomy

摘要: Abstract Tomato is an important agricultural crop and facing severe drought stress. PEG-6000 in five different concentrations was used to artificially induced stress two tomato varieties; Nagina 17905.The results demonstrated that seed germination significantly reduced upto 60% for variety at 8% PEG-6000. Other phenotypic traits such as shoot length, leaf leaves number area also with increasing concentration of PEG-6000, however, root length increased concentration. Fresh biomass both varieties found maximum 1.51 g 2.50 g 2% minimum 1.20 g 1.14 g respectively. However, showed more tolerance than 17,905 concentrations. At molecular level, the three selected genes i.e. SlAREB, SlNCED3, SlERF024 were be over expressed In silico characterization these revealed conserved protein domains, involved These concurred conclude plant could produce tolerant through transformation.

参考文章(53)
Babu Valliyodan, Henry T Nguyen, Understanding regulatory networks and engineering for enhanced drought tolerance in plants. Current Opinion in Plant Biology. ,vol. 9, pp. 189- 195 ,(2006) , 10.1016/J.PBI.2006.01.019
J. Zhang, H. G. Zheng, A. Aarti, G. Pantuwan, T. T. Nguyen, J. N. Tripathy, A. K. Sarial, S. Robin, R. C. Babu, Bay D. Nguyen, S. Sarkarung, A. Blum, H. T. Nguyen, Locating genomic regions associated with components of drought resistance in rice: comparative mapping within and across species Theoretical and Applied Genetics. ,vol. 103, pp. 19- 29 ,(2001) , 10.1007/S001220000534
Dongyun Hao, Masaru Ohme-Takagi, Akinori Sarai, Unique Mode of GCC Box Recognition by the DNA-binding Domain of Ethylene-responsive Element-binding Factor (ERF Domain) in Plant Journal of Biological Chemistry. ,vol. 273, pp. 26857- 26861 ,(1998) , 10.1074/JBC.273.41.26857
K Singh, Transcription factors in plant defense and stress responses. Current Opinion in Plant Biology. ,vol. 5, pp. 430- 436 ,(2002) , 10.1016/S1369-5266(02)00289-3
Bjorn Martin, Charles G. Tauer, Robert K. Lin, Carbon Isotope Discrimination as a Tool to Improve Water‐Use Efficiency in Tomato Crop Science. ,vol. 39, pp. 1775- 1783 ,(1999) , 10.2135/CROPSCI1999.3961775X
Hao Chen, Wei Chen, Junli Zhou, Hang He, Liangbi Chen, Haodong Chen, Xing Wang Deng, None, Basic leucine zipper transcription factor OsbZIP16 positively regulates drought resistance in rice. Plant Science. ,vol. 193, pp. 8- 17 ,(2012) , 10.1016/J.PLANTSCI.2012.05.003
Noorullah Khan, Rashid H. Kazmi, Leo A. J. Willems, Adriaan W. van Heusden, Wilco Ligterink, Henk W. M. Hilhorst, Exploring the Natural Variation for Seedling Traits and Their Link with Seed Dimensions in Tomato PLoS ONE. ,vol. 7, pp. e43991- ,(2012) , 10.1371/JOURNAL.PONE.0043991
Jane Glazebrook, Genes controlling expression of defense responses in Arabidopsis — 2001 status Current Opinion in Plant Biology. ,vol. 4, pp. 301- 308 ,(2001) , 10.1016/S1369-5266(00)00177-1
Hiroshi Nonami, Plant water relations and control of cell elongation at low water potentials Journal of Plant Research. ,vol. 111, pp. 373- 382 ,(1998) , 10.1007/BF02507801
Mitsutomo Abe, Yasushi Kobayashi, Sumiko Yamamoto, Yasufumi Daimon, Ayako Yamaguchi, Yoko Ikeda, Harutaka Ichinoki, Michitaka Notaguchi, Koji Goto, Takashi Araki, FD, a bZIP Protein Mediating Signals from the Floral Pathway Integrator FT at the Shoot Apex Science. ,vol. 309, pp. 1052- 1056 ,(2005) , 10.1126/SCIENCE.1115983