Hyperglycosylation of hen egg white lysozyme in yeast.

作者： S. Nakamura , H. Takasaki , K. Kobayashi , A. Kato

关键词: Site-directed mutagenesis 、 Biochemistry 、 Asparagine 、 Molecular biology 、 Glycoprotein 、 Protein structure 、 Lysozyme 、 Glycosylation 、 Biology 、 Mannose 、 Molecular mass

摘要: … Electrophoresis was carried out at a constant current of 10 mA for 5 h using an electrophoretic buffer of Tris/glycine containing 0.1% SDS. After electrophoresis, the gel sheets were …