Gene cloning and containment properties of plasmid Col E1 and its derivatives

作者: K. Armstrong , V Hershfield , D. Helinski

DOI: 10.1126/SCIENCE.322277

关键词: Strain (chemistry)GeneticsCloningPlasmidEscherichia coliRecombinant DNAMolecular biologyBiologyMolecular cloning

摘要: Colicinogenic plasmid E1 (Col E1) and Col derivatives offer advantages as cloning vehicles with regard to both utility biological containment. The derivative pCR1 does not alter those essential characteristics of the enfeebled Escherichia coli strain x1776 that make this particularly useful a host-vehicle system for recombinant DNA research.

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