Genetic organization of the broad-host-range IncP-1 plasmid R751.
作者： R J Meyer , J A Shapiro
DOI: 10.1128/JB.143.3.1362-1373.1980
关键词:
摘要: We have identified regions encoding conjugal transfer, plasmid maintenance, and trimethoprim resistance on the IncP-1 R751 by complementation tests with cloned deoxyribonucleic acid fragments self-replicating derivatives constructed in vitro. The genes for replication transfer show a scattered organization similar to that previously determined RK2, another plasmid. Derivatives of RK2 are able complement defective these functions. Restriction enzyme cleavage sites clustered physical map. Neither region is required maintenance or although one determines trimethoprim. A clustering seen which nevertheless has very different restriction
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