The Use of Immunological Techniques in Plant Hormone Analysis

作者: A. Crozier , G. Sandberg , A. M. Monteiro , B. Sundberg

DOI: 10.1007/978-3-642-71018-6_2

关键词: BiologyAntiserumAffinity chromatographyPlant hormoneHuman bloodGrowth regulatorHormoneInsulinBiochemistryRadioimmunoassay

摘要: Immunoassays were first used by Berson and Yalow [4] to measure insulin in human blood the technique was subsequently adapted for analysis of a variety compounds involved animal metabolism [1]. Antisera raised against indole-3-acetic acid (IAA) gibberellin A3 initially an assay based on inhibition activation modified bacteriophage T4 [6, 8, 9]. However, these reports received only limited attention it not until development radioimmunoassays (RIA) [2, 10, 14, 18–21, 25] enzyme-linked immunoabsorbent assays (ELISA [3, 5, 22, 24] plant hormones that potential value immunological techniques growth regulator research began be more generally appreciated.

参考文章(27)
J. MacMillan, Molecular aspects of plant hormones Springer-Verlag. ,(1980)
Guy E. Abraham, Handbook of radioimmunoassay Marcel Dekker, Inc.. ,(1977)
P. F. Wareing, Plant growth substances 1982 Plant growth substances 1982.. ,(1982)
Yoram Fuchs, Eitan Gertman, Insoluble antibody column for isolation and quantitative determination of gibberellins Plant and Cell Physiology. ,vol. 15, pp. 629- 633 ,(1974) , 10.1093/OXFORDJOURNALS.PCP.A075047
Sara Fuchs, Joseph Haimovich, Yoram Fuchs, Immunological studies of plant hormones. Detection and estimation by immunological assays. FEBS Journal. ,vol. 18, pp. 384- 390 ,(1971) , 10.1111/J.1432-1033.1971.TB01254.X
Yoram Fuchs, Shimon Mayak, Sara Fuchs, Detection and quantitative determination of abscisic acid by immunological assay. Planta. ,vol. 103, pp. 117- 125 ,(1972) , 10.1007/BF00387362
Björn sundberg, Gōran Sandberg, Alan Crozier, Purification of indole-3-acetic acid in plant extracts by immunoaffinity chromatography Phytochemistry. ,vol. 25, pp. 295- 298 ,(1986) , 10.1016/S0031-9422(00)85469-2
Elmar W. Weiler, Radioimmunoassays for trans-zeatin and related cytokinins Planta. ,vol. 149, pp. 155- 162 ,(1980) , 10.1007/BF00380877