Incorporation and differentiation of hippocampus-derived neural stem cells transplanted in injured adult rat retina.

摘要: Purpose In a previous study it has been shown that adult rat hippocampus-derived neural stem cells can be successfully transplanted into neonatal retinas, where they differentiate neurons and glia, but cannot retinas. the current study, effect of mechanical injury to retina on survival differentiation grafted hippocampal was determined. Methods Mechanical induced in by hooked needle. A cell suspension (containing 90,000 cells) slowly injected vitreous space. The specimens were processed for immunohistochemical studies at 1, 2, 4 weeks after transplantation. Results best case, incorporation seen 50% injured Most these located from ganglion layer through inner nuclear close site. Immunohistochemically, 1 week, more than half expressed nestin. At weeks, some showed immunoreactivity microtubule-associated protein (MAP) 2ab, MAP5, glial fibrillary acidic (GFAP), suggesting progress neuronal astroglial lineages. However, no HPC-1, calbindin, rhodopsin, which suggests did not mature retinal neurons. Immunoelectron microscopy revealed formation synapse-like structures between graft host cells. Conclusions By manipulation injury, subsequent lineage, including structures, achieved, even retina.