Expression of a mitochondrial gene orfH79 from CMS-Honglian rice inhibits Escherichia coli growth via deficient oxygen consumption
作者： Xia Ding , Qiusheng Chen , Canming Bao , Aihua Ai , Ying Zhou
DOI: 10.1186/S40064-016-2822-0
关键词: Dehydrogenase 、 Biology 、 Mitochondrial DNA 、 Pyruvate kinase 、 Chimeric gene 、 Respiration rate 、 Respiration 、 Cytoplasmic male sterility 、 Molecular biology 、 Escherichia coli 、 Biochemistry
摘要: Cytoplasmic male sterility (CMS) has often been associated with abnormal mitochondrial open frames (ORF), orfH79 is a chimeric gene responsible for the CMS trait in Honglian (HL) rice. In this study, weakly produced ORFH79 protein significantly inhibited growth of E. coli an oxygen culture, however, transformants producing was indistinguishable from control under anaerobic incubation conditions. addition, lower respiration rate, wrinkled bacterial surfaces, and decreased pyruvate kinase α-ketoglutarate dehydrogenase activities were observed coli. These results indicate that impairs coli, which may inhibit growth.
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