Rapid plasmid analysis for identification of Edwardsiella ictaluri from infected channel catfish (Ictalurus punctatus).

摘要: Eighteen different strains of Edwardsiella ictaluri isolated from infected channel catfish (Ictalurus punctatus) were screened to determine whether plasmid DNA was present. Two plasmids 5,700 and 4,900 base pairs identified. Restriction enzyme analysis showed that each the harbored these same two plasmids. maps separated indicated not closely related other. A rapid screening technique developed would allow presence either broth cultures or single colonies E. be determined within 2 3 h by agarose gel electrophoresis. These results suggest fingerprinting should become a useful tool in presumptive identification this bacterium catfish. Images