A Novel DNA-Binding Protein with Homology to Myb Oncoproteins Containing Only One Repeat Can Function as a Transcriptional Activator
作者： N. Baranowskij , C. Frohberg , S. Prat , L. Willmitzer
DOI: 10.1002/J.1460-2075.1994.TB06873.X
关键词: DNA binding site 、 MYB 、 Molecular biology 、 HMG-box 、 Biology 、 Binding site 、 Sequence alignment 、 Proto-Oncogene Proteins c-myb 、 DNA-binding domain 、 DNA
摘要: A cDNA clone encoding a novel Myb-related protein, designated MybSt1, was isolated from potato expression library by South Western screening using the CaMV 35S promoter domain as probe. Sequence comparison shows small region with some homology to highly conserved DNA binding of c-myb proto-oncogene consisting three imperfect repeats. The Myb motif MybSt1 protein is distinct plant described so far. In contrast known proteins, two repeats required for activity, mybSt1 contains only one such repeat. Nevertheless, able bind in sequence-specific manner. addition Myb-like region, an acidic segment its central well proline-rich near C-terminus. Applying random site selection technique, high-affinity sites were identified, sharing core GGATA. transient assays protoplasts, clear evidence obtained this myb functioning transcriptional activator.
mpg.de
sci-hub.se 参考文章(62)
U. Schindler, W. Terzaghi, H. Beckmann, T. Kadesch, A.R. Cashmore, DNA binding site preferences and transcriptional activation properties of the Arabidopsis transcription factor GBF1. The EMBO Journal. ,vol. 11, pp. 1275- 1289 ,(1992) , 10.1002/J.1460-2075.1992.TB05171.X
R. Ohi, D. McCollum, B. Hirani, G.J. Den Haese, X. Zhang, J.D. Burke, K. Turner, K.L. Gould, The Schizosaccharomyces pombe cdc5+ gene encodes an essential protein with homology to c-Myb. The EMBO Journal. ,vol. 13, pp. 471- 483 ,(1994) , 10.1002/J.1460-2075.1994.TB06282.X
J. Paz-Ares, D. Ghosal, U. Wienand, P. A. Peterson, H. Saedler, The regulatory c1 locus of Zea mays encodes a protein with homology to myb proto-oncogene products and with structural similarities to transcriptional activators. The EMBO Journal. ,vol. 6, pp. 3553- 3558 ,(1987) , 10.1002/J.1460-2075.1987.TB02684.X
F Kalkbrenner, K Moelling, S Guehmann, Transcriptional activation by human c-myb and v-myb genes. Oncogene. ,vol. 5, pp. 657- 661 ,(1990)
K.M. Howe, C.F. Reakes, R.J. Watson, Characterization of the sequence-specific interaction of mouse c-myb protein with DNA. The EMBO Journal. ,vol. 9, pp. 161- 169 ,(1990) , 10.1002/J.1460-2075.1990.TB08092.X
Philip N. Benfey, Ling Ren, Nam-Hai Chua, The CaMV 35S enhancer contains at least two domains which can confer different developmental and tissue-specific expression patterns. The EMBO Journal. ,vol. 8, pp. 2195- 2202 ,(1989) , 10.1002/J.1460-2075.1989.TB08342.X
R.W. Sablowski, E. Moyano, F.A. Culianez-Macia, W. Schuch, C. Martin, M. Bevan, A flower-specific Myb protein activates transcription of phenylpropanoid biosynthetic genes. The EMBO Journal. ,vol. 13, pp. 128- 137 ,(1994) , 10.1002/J.1460-2075.1994.TB06242.X
A. Kispert, B.G. Herrmann, The Brachyury gene encodes a novel DNA binding protein. The EMBO Journal. ,vol. 12, pp. 3211- 3220 ,(1993) , 10.1002/J.1460-2075.1993.TB05990.X
Q D Ju, B E Morrow, J R Warner, REB1, a yeast DNA-binding protein with many targets, is essential for growth and bears some resemblance to the oncogene myb. Molecular and Cellular Biology. ,vol. 10, pp. 5226- 5234 ,(1990) , 10.1128/MCB.10.10.5226
F. William Studier, Alan H. Rosenberg, John J. Dunn, John W. Dubendorff, Use of T7 RNA polymerase to direct expression of cloned genes. Methods in Enzymology. ,vol. 185, pp. 60- 89 ,(1990) , 10.1016/0076-6879(90)85008-C