The quantitation of IgG4 antibodies to three common food allergens by ELISA with monoclonal anti-IgG4
作者： G.T. Layton , D.R. Stanworth
DOI: 10.1016/0022-1759(84)90410-1
关键词: Gluten 、 Monoclonal 、 Allergen 、 Subclass 、 Immunology 、 Monoclonal antibody 、 Chemistry 、 Ovalbumin 、 Antibody 、 Allergy
摘要: Abstract Enzyme-linked immunosorbent assays, capable of detecting nanogram quantities IgG4 to 3 common food allergens, have been developed with use a monoclonal anti-human IgG4. Calibrated positive human sera were used construct standard curves. The allergens employed, major protein constituents egg (ovalbumin), milk (β-lactoglobulin) and-wheat (gluten), highly effective in coating microtitre plate wells; and the assays proved be sensitive, reproducible specific, terms both allergen IgG subclass. On testing from group non-allergic individuals high proportion found contain antibodies allergens. A pool 60 obtained blood donors also contained all results illustrate that against are frequently encountered normal individuals; this needs taken into consideration assessing possible role such immediate hypersensitivity reactions.
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