Production of a polyhydroxyalkanoate biopolymer in insect cells with a modified eucaryotic Fatty Acid synthase.
作者： Mark D. Williams , John A. Rahn , Stuart Smith , Anil K. Joshi , David H. Sherman
DOI: 10.1128/AEM.64.1.383-383.1998
关键词: Gene product 、 Complementary DNA 、 Biochemistry 、 Enzyme 、 Gene expression 、 Metabolic engineering 、 Biology 、 Fatty acid synthase 、 Mutant 、 Spodoptera
摘要: A novel pathway for the synthesis of poly-3-hydroxybutyrate has been engineered by simultaneous delivery two genes into insect cells (Spodoptera frugiperda) use individual baculovirus vectors. This system includes expression a dehydrase-domain mutant rat fatty acid synthase cDNA and phbC gene encoding polyhydroxyalkanoate from Alcaligenes eutrophus. The dehydrase-deficient provides de novo R-(-)-3-hydroxybutyryl-coenzyme as premature termination product rather than palmityl-coenzyme A, normal wild-type synthase. High levels this multifunctional protein provide suitable precursor pool conversion to in coexpressing product. strategy redesigning biosynthetic suggests new method generating structurally diverse polyhydroxyalkanoates metabolic engineering.
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