The peroxidase-catalyzed oxidation of NADH as an indicator reaction for repetitive determinations by sample injection in closed flow-through systems: The determination of LDH in blood serum

摘要: Abstract The air oxidation of reduced nicotinamide adenine dinucleotide (NADH), catalyzed by peroxidase, provides a useful “indicator reaction” for the determination both NADH and serum lactate dehydrogenase (LDH). Application this indicator reaction repetitive determinations sample injection into continuously circulated reagent mixture, monitoring oxygen depletion with an amperometric sensor, is described. At rates 260 determinations/h, LDH have been determined in range 3.8 × 10−4 to 7.6 10−3 m 70 700 U/ml, respectively, relative errors standard deviations (population) about 2%. has human blood procedure compared modified Wroblewski-Ladue procedure; Pearson correlation coefficient was found be 0.999 (40 samples). Recovery studies are also reported calibration references controls.