Dietary ascorbic acid reduced micronucleus and nuclear abnormalities in Clarias gariepinus (Burchell 1822) exposed to hospital effluent

摘要: Hospital effluents contain myriad of mutagens and genotoxins capable increasing DNA damage in aquatic biota. African mudfish, Clarias gariepinus, are exposed to when cultured swamps derelict water bodies often contaminated by effluents. Moreover, its is susceptible xenobiotic-induced lesions since it lacks l-gulonolactone oxidase hence cannot synthesize l-ascorbic acid. This study investigated 96-h acute toxicity protective effects dietary ascorbic acid (AA) against micronucleus (MN) abnormal nuclear (NAs) formation C. gariepinus sub-lethal concentrations hospital effluent. Six (0.5–3.0%) the effluent were selected determine after range finding test. Fish (0.08–1.30%) 96 h LC50. Two other groups LC50 (1.30%) +50 +100 mg/kg for 7 days, MN NAs assessed peripheral erythrocytes. The was 1.18 times more toxic than 24 h (1.54%), indicating that increased with exposure duration. MN, bud, enucleated, fragmented nucleus (apoptosis), necrotic erythrocytes significantly increase treated fish. Dietary AA reduced from 6.35-fold (1.30% group) 3.72-fold (1.30% + 50 mg AA) 3.54-fold (1.30% + 100 mg AA). Also, total 2.26-fold 1.40-fold 1.06-fold compared control. Heavy metals physicochemical parameters analyzed tested possibly induced mortality cytogenotoxicity this ameliorated AA.