A single-nucleotide exon found in Arabidopsis
作者： Lei Guo , Chun-Ming Liu
DOI: 10.1038/SREP18087
关键词: Exonic splicing enhancer 、 Intron 、 Exon 、 Exon shuffling 、 Genetics 、 RNA splicing 、 Splice site mutation 、 Biology 、 Group II intron 、 Exon trapping
摘要: The presence of introns in gene-coding regions is one the most mysterious evolutionary inventions eukaryotic organisms. It has been proposed that, although sequences involved intron recognition and splicing are mainly located introns, exonic also contribute to splicing. smallest constitutively spliced exon known so far 6 nucleotides, alternatively 3 nucleotides. Here we report that Anaphase Promoting Complex subunit 11 (APC11) gene Arabidopsis thaliana carries a constitutive single-nucleotide exon. In vivo transcription translation assays performed using APC11-Green Fluorescence Protein (GFP) fusion constructs revealed surrounding effective both rice. This discovery warrants attention genome annotations future.
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