Genome-wide identification of zero nucleotide recursive splicing in Drosophila

摘要: Recursive splicing is a process in which large introns are removed multiple steps by re-splicing at ratchet points--5' splice sites recreated after splicing. was first identified the Drosophila Ultrabithorax (Ubx) gene and only three additional genes have since been experimentally shown to undergo recursive Here we identify 197 zero nucleotide exon points 130 of 115 from total RNA sequencing data generated developmental time points, dissected tissues cultured cells. The sequential nature confirmed identification lariat points. We also show that constitutive process, depletion U2AF inhibits splicing, sequence function evolutionarily conserved Drosophila. Finally, four recursively spliced human genes, one Together, these results indicate commonly used Drosophila, occurs humans, provides insight into mechanisms some removed.