Droplet Digital PCR Based Androgen Receptor Variant 7 (AR-V7) Detection from Prostate Cancer Patient Blood Biopsies.
作者： Yafeng Ma , Alison Luk , Francis Young , David Lynch , Wei Chua
DOI: 10.3390/IJMS17081264
关键词: Prostate cancer 、 Polymerase chain reaction 、 PCA3 、 Digital polymerase chain reaction 、 Biomarker (medicine) 、 Biology 、 Oncology 、 Internal medicine 、 Epithelial cell adhesion molecule 、 Androgen receptor 、 Circulating tumor cell
摘要: Androgen receptor splice variant V7 (AR-V7) was recently identified as a valuable predictive biomarker in metastatic castrate-resistant prostate cancer. Here, we report new, sensitive and accurate screen for AR-V7 mRNA expression directly from circulating tumor cells (CTCs): We combined EpCAM-based immunomagnetic CTC isolation using the IsoFlux microfluidic platform with droplet digital polymerase chain reaction (ddPCR) to analyze total AR cancer patients CTCs. demonstrate that is reliably detectable enriched samples little five CTCs, even considering heterogeneity, confirm detection of advanced (PCa) limited castrate resistant disease status our sample set. Sensitive molecular analyses (CTCs) or nucleic acids present exciting strategies detect biomarkers, such non-invasive blood samples, so-called biopsies.
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Wendy Onstenk, Anieta M. Sieuwerts, Jaco Kraan, Mai Van, Annemieke J.M. Nieuweboer, Ron H.J. Mathijssen, Paul Hamberg, Hielke J. Meulenbeld, Bram De Laere, Luc Y. Dirix, Robert J. van Soest, Martijn P. Lolkema, John W.M. Martens, Wytske M. van Weerden, Guido W. Jenster, John A. Foekens, Ronald de Wit, Stefan Sleijfer, Efficacy of Cabazitaxel in Castration-resistant Prostate Cancer Is Independent of the Presence of AR-V7 in Circulating Tumor Cells European Urology. ,vol. 68, pp. 939- 945 ,(2015) , 10.1016/J.EURURO.2015.07.007
Maria Gonzalez-Pons, Marcia Cruz-Correa, Colorectal Cancer Biomarkers: Where Are We Now? BioMed Research International. ,vol. 2015, pp. 149014- 149014 ,(2015) , 10.1155/2015/149014
C. Pezaro, H. H. Woo, I. D. Davis, Prostate cancer: measuring PSA Internal Medicine Journal. ,vol. 44, pp. 433- 440 ,(2014) , 10.1111/IMJ.12407
Emmanuel S Antonarakis, Changxue Lu, Brandon Luber, Hao Wang, Yan Chen, Mary Nakazawa, Rosa Nadal, Channing J Paller, Samuel R Denmeade, Michael A Carducci, Mario A Eisenberger, Jun Luo, None, Androgen Receptor Splice Variant 7 and Efficacy of Taxane Chemotherapy in Patients With Metastatic Castration-Resistant Prostate Cancer JAMA Oncology. ,vol. 1, pp. 582- 591 ,(2015) , 10.1001/JAMAONCOL.2015.1341
Julie Steinestel, Manuel Luedeke, Annette Arndt, Thomas J. Schnoeller, Jochen K. Lennerz, Carina Wurm, Christiane Maier, Marcus V. Cronauer, Konrad Steinestel, Andres J. Schrader, Detecting predictive androgen receptor modifications in circulating prostate cancer cells Oncotarget. ,vol. 10, pp. 4213- 4223 ,(2015) , 10.18632/ONCOTARGET.3925
Harri Makkonen, Miia Kauhanen, Tiina Jääskeläinen, Jorma J. Palvimo, Androgen receptor amplification is reflected in the transcriptional responses of Vertebral-Cancer of the Prostate cells Molecular and Cellular Endocrinology. ,vol. 331, pp. 57- 65 ,(2011) , 10.1016/J.MCE.2010.08.008
Yuanyuan Qu, Bo Dai, Dingwei Ye, Yunyi Kong, Kun Chang, Zhongwei Jia, Xiaoqun Yang, Hailiang Zhang, Yao Zhu, Guohai Shi, Constitutively Active AR-V7 Plays an Essential Role in the Development and Progression of Castration-Resistant Prostate Cancer Scientific Reports. ,vol. 5, pp. 7654- 7654 ,(2015) , 10.1038/SREP07654
Yuqiu Jiang, John F Palma, David B Agus, Yixin Wang, Mitchell E Gross, Detection of Androgen Receptor Mutations in Circulating Tumor Cells in Castration-Resistant Prostate Cancer Clinical Chemistry. ,vol. 56, pp. 1492- 1495 ,(2010) , 10.1373/CLINCHEM.2010.143297
Yongjiang Yu, Yu Zhang, Wenbin Guan, Tao Huang, Jian Kang, Xujun Sheng, Jun Qi, Androgen receptor promotes the oncogenic function of overexpressed Jagged1 in prostate cancer by enhancing cyclin B1 expression via Akt phosphorylation. Molecular Cancer Research. ,vol. 12, pp. 830- 842 ,(2014) , 10.1158/1541-7786.MCR-13-0545
Maria Thadani-Mulero, Luigi Portella, Shihua Sun, Matthew Sung, Alexandre Matov, Robert L. Vessella, Eva Corey, David M. Nanus, Stephen R. Plymate, Paraskevi Giannakakou, Androgen Receptor Splice Variants Determine Taxane Sensitivity in Prostate Cancer Cancer Research. ,vol. 74, pp. 2270- 2282 ,(2014) , 10.1158/0008-5472.CAN-13-2876