Scaffolded fusion polypeptides and compositions and methods for making the same

作者: Timothy A. Coleman , Brian C. Mansfield

DOI:

关键词: ChemistryBiological activityFusionBiochemistryIntegral membrane protein

摘要: The present invention provides soluble forms of integral membrane proteins, or domains portions thereof, that retain the biological activity protein, domain portion from which they are designed derived and can readily be expressed in high yield.

参考文章(29)
Beata Lecka-Czernik, DNA binding protein S1-3 ,(1996)
Barbara Imperiali, Grant K. Walkup, Peptidyl fluorescent chemosensor for divalent zinc ,(1997)
Robert D. Radcliffe, Thomas J. Mckearn, John D. Rodwell, Vernon L. Alvarez, Molecular recognition units ,(1991)
David Goldman, Markku Linnoila, Jaakko Lappalainen, Allelic variation of the serotonin 5ht2c receptor ,(1995)
Alan Fersht, Jean Chatellier, Fergal Conan Hill, Protein scaffold and its use to multimerise monomeric polypeptides ,(2000)
Isidro Sanchez-Garcia, Yen Choo, Aaron Klug, Binding proteins for recognition of DNA ,(1995)
Hyeryun Choe, Michael Farzan, Ying Sun, Nancy Sullivan, Barrett Rollins, Paul D Ponath, Lijun Wu, Charles R Mackay, Gregory LaRosa, Walter Newman, Norma Gerard, Craig Gerard, Joseph Sodroski, The β-Chemokine Receptors CCR3 and CCR5 Facilitate Infection by Primary HIV-1 Isolates Cell. ,vol. 85, pp. 1135- 1148 ,(1996) , 10.1016/S0092-8674(00)81313-6
Andrew D Howard, George McAllister, Scott D Feighner, Qingyun Liu, Ravi P Nargund, Lex H.T Van der Ploeg, Arthur A Patchett, Orphan G-protein-coupled receptors and natural ligand discovery Trends in Pharmacological Sciences. ,vol. 22, pp. 132- 140 ,(2001) , 10.1016/S0165-6147(00)01636-9
Zixuan Wang, Benhur Lee, James L. Murray, Fabien Bonneau, Yi Sun, Vicki Schweickart, Tianyuan Zhang, Stephen C. Peiper, CCR5 HIV-1 coreceptor activity. Role of cooperativity between residues in N-terminal extracellular and intracellular domains Journal of Biological Chemistry. ,vol. 274, pp. 28413- 28419 ,(1999) , 10.1074/JBC.274.40.28413