An agarose gel-based neurosphere culture system leads to enrichment of neuronal lineage cells in vitro

摘要: Stem cell-based therapy holds great potential especially for neurological disorders. However, clinical applications await further understanding of many aspects stem cell differentiation and development technology enabling manipulation cells into desired types in the central nervous system. Here, we developed a new method that leads to enrichment neuronal lineage neural cultures. The protocol involves cultivation primary derived from forebrains rat E18 embryos above layer nonadhesive hard agarose gel form neurospheres. In contrast neurospheres were cultured an anti-adhesive hydrogel layer, preferentially differentiated β-III tubulin-positive neurons when allowed undergo vitro.In effort investigate mechanism behind this observation, found gene expression vertebrate determination (neurogenin1) was enhanced proliferated as compared with control, level neurogenin1 quite well correlated rigidity gel. These results indicate can contribute, at least part, enrich progenitors immature postmitotic during neurosphere formation may provide additional information establish efficient protocols study.