Production of neurons, astrocytes and oligodendrocytes from mammalian CNS stem cells

摘要: The isolation and expansion of precursor cells in a serum-free culture system allows for the systematic characterization their properties intrinsic extrinsic signals that regulate function. discovery neural stem adult mouse brain was made possible by creation novel subsequently termed neurosphere assay. Therein, dissociated periventricular area plated presence epidermal growth factor, but absence adhesive substrates, which resulted generation spheres proliferating detached from plate bottom remained suspended media. Since its inception, has been widely used cell field extensively adapted corneal, cardiac, skin, prostate, mammary tumor cells. original protocol, takes approximately 10 d to complete, is described here detail.