The role of Fas/APO 1 and apoptosis in the development of human atherosclerotic lesions

摘要: The possibility that Fas/APO 1 is involved in the apoptosis of advanced human coronary atherosclerosis was examined present study. Coronary arteries with were obtained from hearts chronic ischemic heart disease at cardiac transplantation. Normal vessels used as controls. detected by immunohistochemistry a monoclonal antibody. Apoptotic cells stained situ terminal deoxynucleotidyl transferase mediated-dUTP nick end labeling (TUNEL) and DNA fragmentation into oligonucleosomes checked gel electrophoresis. Bcl-2, an antiapoptotic oncoprotein, Western blot. neointima all stages atherosclerosis, intraplaque small vessels. In initial lesions, only few undergoing apoptosis. By contrast, many found to undergo Apoptosis further confirmed genomic analysis using either smooth muscle or macrophages, but also endothelial blood borne cells. foam Most positive for macrophage marker CD68 α-smooth actin serial sections. Several anti-Fas/APO revealed double staining. Bcl-2 expressing plaques. A number CD3-positive T-lymphocytes around 1. This data suggests regulated development atherosclerotic lesions it probably determines amount tissue mass diseased