Central cholinergic system mediates working memory deficit induced by anesthesia/surgery in adult mice.

摘要: Background Postoperative cognitive dysfunction (POCD) is consistently associated with increased morbidity and mortality, which has become a major concern of patients caregivers. Although POCD occurs mainly in aged patients, it happens at any age. Previous studies demonstrated that anesthesia/surgery had no effects on reference memory adult mice. However, whether impairs working remains unclear. Working deficit would result many deficits executive function. We hypothesized impaired the mice central cholinergic system was involved. Method Tibial fracture internal fixation under anesthesia isoflurane performed two-month-old C57BL/6 Two days later, spatial were measured by Morris Water Maze (MWM). Donepezil, an inhibitor acetylcholinesterase (AChE), administered another cohort for 4 weeks. Then, MWM 2 days after anesthesia/surgery. Western blot used to detect protein levels acetylcholine transferase (ChAT), AChE, vesicular transporter (VAChT), choline (ChT) prefrontal cortex (PFC). Results found memory, but Meanwhile, we also level ChAT PFC decreased significantly compared control group. Donepezil pretreatment prevented impairment decrease induced Conclusion These results suggest leads