Immunohistochemical localization of basic fibroblast growth factor in wound healing sites of mouse skin

摘要: The immunohistochemical localization of basic fibroblast growth factor (bFGF) was examined during wound healing in mouse skin. Frozen sections taken from the rounded skin defects were reacted with polyclonal anti-human recombinant bFGF IgG followed by incubation FITC-conjugated IgG. basal layer keratinocytes and hair bulbs at edge strongly stained this antibody. In reepithelized area, several layers positively regardless time after wounding. These findings suggest that germinative which express function as leading cells covering defect. However, dermal granulation tissue, including capillary endothelial cells, fibroblasts macrophages unexpectedly did not demonstrate any immunoreactivity throughout process healing. Simultaneous histochemical investigation using cultivated bovine aortic showed primarily cytoplasmic fluorescence. discrepancy staining patterns vivo vitro suggests immunoreactive is either expressed vivo, or processed masked.