Polyspecific natural antibodies and autoantibodies secreted by human lymphocytes immortalized with Epstein-Barr virus

摘要: Recent studies have shown that autoreactive B cells and autoantibodies are present in pathological as well normal situations. In the study, we immortalized human cell lines from individuals patients with malignant or benign dysglobulinemia Epstein-Barr virus examined, after cloning, autoantibody reactivities of immunoglobulins secreted by these cells. Forty-two supernatants were analyzed enzyme-immunoassay on a panel 13 self non-self antigens: trinitrobenzenesulfonic acid (TNP), DNA, L- glutamine, L-alanine, L-tyrosine (GAT), actin, myosin, tubulin, albumin, renin, spectrin, transferrin, thyroglobulin, myoglobin, peroxidase, immunofluorescence tissue sections. Fourteen (33%) immunoglobulin-secreting found to an function; seven IgM, six IgA, one IgG. The light chains kappa type 11 cases. vast majority clones reacted more than five antigens all them TNP. No correlation was between given isotype antibody specificity. More half antibodies also cellular None four secreting monoclonal antiviral any panel. results indicate lymphoid can polyspecific functions, similar those polyclonal antibodies, paraproteins natural synthesized murine rat obtained physiologically animals.