Activation of O2−‐generating oxidase in an heterologous cell‐free system derived from Epstein‐Barr‐virus‐transformed human B lymphocytes and bovine neutrophils
作者： Laurence COHEN-TANUGI , Francoise MOREL , Marie-Claire PILLOUD-DAGHER , Jean Marie SEIGNEURIN , Patrice FRANCOIS
DOI: 10.1111/J.1432-1033.1991.TB16419.X
关键词:
摘要: Epstein-Barr-virus-transformed human B lymphocytes (EBV lymphocytes) stimulated by 4β-phorbol 12-myristate 13-acetate exhibit an NADPH-dependent oxidase activity capable of generating the superoxide anion O2−, similar to, but less efficient than that activated neutrophils. A cell-free system activation consisting a membrane fraction and cytosol from EBV lymphocyte homogenate supplemented with guanosine 5′-[γ-thio]triphosphate (GTP[S]), arachidonic acid Mg2+ was found to be competent in production assessed superoxide-dismutase-sensitive reduction cytochrome c presence NADPH. However, slow largely insensitive both dismutase, iodonium biphenyl, powerful inhibitor markedly faster eduction NADPh obtained heterologous bovine neutrophil membranes, GTP[S], Mg2+; this system, totally inhibited dismutase biphenyl. These results show contain substantial amount cytosolic factors activation, limiting for O2− are components complex. The membranes provided rapid convenient method diagnose defects autosomal forms chronic granulomatous disease. In addition, it might useful tool explore mechanism action activation.
sci-hub.se 参考文章(24)
J T Hancock, F E Maly, O T Jones, Properties of the superoxide-generating oxidase of B-lymphocyte cell lines. Determination of Michaelis parameters. Biochemical Journal. ,vol. 262, pp. 373- 375 ,(1989) , 10.1042/BJ2620373
J. T. Hancock, O. T. G. Jones, L. M. Henderson, Superoxide generation by EBV-transformed B lymphocytes. Activation by IL-1 beta, TNF-alpha and receptor independent stimuli. Immunology. ,vol. 71, pp. 213- 217 ,(1990)
Marco Baggiolini, Walter Ruch, Philip H. Cooper, Measurement of hydrogen peroxide production by phagocytes using homovanillic acid and horseradish peroxidase. Methods in Enzymology. ,vol. 132, pp. 395- 400 ,(1986) , 10.1016/S0076-6879(86)32024-X
A S Fauci, J I Gallin, D J Volkman, E S Buescher, B cell lines as models for inherited phagocytic diseases: abnormal superoxide generation in chronic granulomatous disease and giant granules in Chediak-Higashi syndrome. Journal of Immunology. ,vol. 133, pp. 3006- 3009 ,(1984)
JM Seigneurin, B Guilbert, MJ Bourgeat, S Avrameas, Polyspecific natural antibodies and autoantibodies secreted by human lymphocytes immortalized with Epstein-Barr virus Blood. ,vol. 71, pp. 581- 585 ,(1988) , 10.1182/BLOOD.V71.3.581.581
C M Yea, A R Cross, O T G Jones, Purification and some properties of the 45 kDa diphenylene iodonium-binding flavoprotein of neutrophil NADPH oxidase. Biochemical Journal. ,vol. 265, pp. 95- 100 ,(1990) , 10.1042/BJ2650095
G. Wolf-Vorbeck, A. L. De Weck, C. A. Dahinden, A. R. Cross, C. Walker, O. T. G. Jones, F. E. Maly, The superoxide generating system of B cell lines. Structural homology with the phagocytic oxidase and triggering via surface Ig. Journal of Immunology. ,vol. 140, pp. 2334- 2339 ,(1988)
Francoise MOREL, Jacques DOUSSIERE, Marie-Jose STASIA, Pierre V. VIGNAIS, The respiratory burst of bovine neutrophilis FEBS Journal. ,vol. 152, pp. 669- 679 ,(1985) , 10.1111/J.1432-1033.1985.TB09247.X
Francois LAPORTE, Jacques DOUSSIERE, Pierre V. VIGNAIS, Respiratory burst of rabbit peritoneal neutrophils FEBS Journal. ,vol. 194, pp. 301- 308 ,(1990) , 10.1111/J.1432-1033.1990.TB19457.X
A L de Weck, C A Dahinden, A Urwyler, J F Gauchat, A R Cross, C Walker, O T Jones, M Nakamura, F E Maly, Superoxide-dependent nitroblue tetrazolium reduction and expression of cytochrome b-245 components by human tonsillar B lymphocytes and B cell lines. Journal of Immunology. ,vol. 142, pp. 1260- 1267 ,(1989)