Identifying potential biomarkers related to pre-term delivery by proteomic analysis of amniotic fluid.
作者： Subeen Hong , Ji Eun Lee , Yu Mi Kim , Yehyon Park , Ji-Woong Choi
DOI: 10.1038/S41598-020-76748-1
关键词: Gestational age 、 Amniocentesis 、 Case-control study 、 Andrology 、 Cohort 、 Pregnancy 、 Amniotic fluid 、 Medicine 、 Retrospective cohort study 、 Gestation
摘要: We sought to identify biomarkers in the amniotic fluid (AF) and specific signaling pathways related spontaneous preterm delivery (SPTD, < 34 weeks) women with labor (PTL) without intra-uterine infection/inflammation (IUI). This was a retrospective cohort study of total 139 PTL singleton gestation (24 + 0 32 6 who underwent amniocentesis displayed no evidence IUI. A nested case-control conducted using pooled AF samples (n = 20) analyzed via label-free liquid chromatography-tandem mass spectrometry. In cohort, an ELISA validation performed for seven candidate proteins interest. Proteomic analysis identified 77 differentially expressed (DEPs, P 0.05) from SPTD cases compared term controls. confirmed that had before weeks significantly independently lower levels VEGFR-1 higher lipocalin-2 Fc fragment IgG binding protein AF. Five principle associated DEPs were identified, including glycolysis, gluconeogenesis, iron homeostasis. The proteomic data AFs several novel absence
nature.com 
sci-hub.st 参考文章(55)
Lisa M. Christian, Effects of stress and depression on inflammatory immune parameters in pregnancy American Journal of Obstetrics and Gynecology. ,vol. 211, pp. 275- 277 ,(2014) , 10.1016/J.AJOG.2014.06.042
Elizabeth Ramsey Unal, Jill T. Cierny, Chantel Roedner, Roger Newman, Laura Goetzl, Maternal inflammation in spontaneous term labor American Journal of Obstetrics and Gynecology. ,vol. 204, pp. 223.e1- 223.e5 ,(2011) , 10.1016/J.AJOG.2011.01.002
Marie Vajrychova, Marian Kacerovsky, Vojtech Tambor, Helena Hornychova, Juraj Lenco, Microbial invasion and histological chorioamnionitis upregulate neutrophil-gelatinase associated lipocalin in preterm prelabor rupture of membranes Journal of Maternal-fetal & Neonatal Medicine. ,vol. 29, pp. 12- 21 ,(2016) , 10.3109/14767058.2014.991305
Sung Youn Lee, Kyo Hoon Park, Eun Ha Jeong, Kyung Joon Oh, Aeli Ryu, Ahra Kim, Intra-amniotic infection/inflammation as a risk factor for subsequent ruptured membranes after clinically indicated amniocentesis in preterm labor. Journal of Korean Medical Science. ,vol. 28, pp. 1226- 1232 ,(2013) , 10.3346/JKMS.2013.28.8.1226
Roberto Romero, Ricardo Gomez, Tinnakorn Chaiworapongsa, Giancarlo Conoscenti, Ju Cheol Kim, Yeon Mee Kim, The role of infection in preterm labour and delivery. Paediatric and Perinatal Epidemiology. ,vol. 15, pp. 41- 56 ,(2001) , 10.1046/J.1365-3016.2001.00007.X
Mozammel H. Gazi, MeiLan He, John C. Cheville, Charles Y.F. Young, Downregulation of IgG Fc binding protein (FcγBP) in prostate cancer Cancer Biology & Therapy. ,vol. 7, pp. 70- 75 ,(2008) , 10.4161/CBT.7.1.5131
Yong-Yook Lee, Kimberly Q McKinney, Sriparna Ghosh, David A Iannitti, John B Martinie, F Ryan Caballes, Mark W Russo, William A Ahrens, Deborah H Lundgren, David K Han, Herbert L Bonkovsky, Sun-Il Hwang, None, Subcellular Tissue Proteomics of Hepatocellular Carcinoma for Molecular Signature Discovery Journal of Proteome Research. ,vol. 10, pp. 5070- 5083 ,(2011) , 10.1021/PR2005204
Lincon A. Stamp, David R. Braxton, Jun Wu, Veronika Akopian, Kouichi Hasegawa, Parakrama T. Chandrasoma, Susan M. Hawes, Catriona McLean, Lydia M. Petrovic, Kasper Wang, Martin F. Pera, The GCTM-5 epitope associated with the mucin-like glycoprotein FCGBP marks progenitor cells in tissues of endodermal origin. Stem Cells. ,vol. 30, pp. 1999- 2009 ,(2012) , 10.1002/STEM.1167
Phillip C. Greig, J.M. Ernest, Lisa Teot, Low amniotic fluid glucose levels are a specific but not a sensitive marker for subclinical intrauterine infections in patients in preterm labor with intact membranes American Journal of Obstetrics and Gynecology. ,vol. 171, pp. 365- 371 ,(1994) , 10.1016/S0002-9378(94)70036-2
Catherine Y. Spong, Alessandro Ghidini, Miguel Ossandon, Charles N. Walker, John C. Pezzullo, Are the cytokines interleukin-6 and angiogenin stable in frozen amniotic fluid? American Journal of Obstetrics and Gynecology. ,vol. 178, pp. 783- 786 ,(1998) , 10.1016/S0002-9378(98)70492-2