Subcellular Tissue Proteomics of Hepatocellular Carcinoma for Molecular Signature Discovery
作者： Yong-Yook Lee , Kimberly Q McKinney , Sriparna Ghosh , David A Iannitti , John B Martinie
DOI: 10.1021/PR2005204
关键词:
摘要: Hepatocellular carcinoma (HCC) is one of the leading causes mortality from solid organ malignancy worldwide. Because complexity proteins within liver cells and tissues, discovery therapeutic targets HCC has been difficult. To investigate strategies for decreasing tissue samples detecting meaningful protein mediators HCC, we employed subcellular fractionation combined with 1D-gel electrophoresis liquid chromatography–tandem mass spectrometry analysis. Moreover, utilized a statistical method, namely, Power Law Global Error Model (PLGEM), to distinguish differentially expressed in duplicate proteomic data set. Mass spectrometric analysis identified 3045 nontumor cytosolic, membrane, nuclear, cytoskeletal fractions. The final lists highly differentiated targeted fractions were searched potentially translocated soluble compartments nuclear or compa...
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