Pyridoxine 5'-phosphate oxidase is correlated with human breast invasive ductal carcinoma development.
作者： Weimin Ren , Wencai Guan , Jinguo Zhang , Fanchen Wang , Guoxiong Xu
关键词: Cancer research 、 Gene knockdown 、 PNPO 、 microRNA 、 Apoptosis 、 MALAT1 、 Pyridoxine 5'-phosphate oxidase 、 Metastasis 、 Cell cycle 、 Biology
摘要: Pyridoxine 5'-phosphate oxidase (PNPO) is a converting enzyme for an active form of vitamin B6. This study aims to evaluate the biological function and regulatory mechanism PNPO in human breast invasive ductal carcinoma (IDC). We unveiled first time that was upregulated patients with IDC correlated overall survival metastasis at later stages. Suppression inhibited cancer cell proliferation, migration, invasion colony formation, arrested cycle G2/M phase induced apoptosis. positively lncRNA MALAT1 which negatively miR-216b-5p. down-regulated up-regulated by miR-216b-5p mimics inhibitors, respectively, cells. A microRNA response element found both transcripts dual-luciferase reporter assay confirmed binding these transcripts. Knockdown resulted increase decrease mRNA, indicating competing endogenous RNAs. Taken together, results reveal PNPO, MALAT1/miR-216b-5p/PNPO axis may be important development. Targeting this have therapeutic potential cancer.
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