Role of the Alanine at Position 363 of Cytochrome P450 2B2 in Influencing the NADPH- and Hydroperoxide-Supported Activities☆☆☆

作者: Imad H Hanna , John F Teiber , Kristin L Kokones , Paul F Hollenberg

DOI: 10.1006/ABBI.1997.0534

关键词: BiochemistryBenzphetamineStereochemistryCytochrome P450Cytochrome b5MicrosomeCumene hydroperoxideHydroxylationDMBAMutantChemistry

摘要: … of the amino acid 2 wild-type enzymes (16). Both mutations affected the substitutions with respect to the postulated … With the A363V recognition sites of the P450 family 2 enzymes (17). …

参考文章(45)
K.M. Kedzie, C.A. Balfour, G.Y. Escobar, S.W. Grimm, Y.A. He, D.J. Pepperl, J.W. Regan, J.C. Stevens, J.R. Halpert, Molecular basis for a functionally unique cytochrome P450IIB1 variant. Journal of Biological Chemistry. ,vol. 266, pp. 22515- 22521 ,(1991) , 10.1016/S0021-9258(18)54602-0
F. Peter Guengerich, Martha V. Martin, Zuyu Guo, Young-Jin Chun, PURIFICATION OF FUNCTIONAL RECOMBINANT P450S FROM BACTERIA Methods in Enzymology. ,vol. 272, pp. 35- 44 ,(1996) , 10.1016/S0076-6879(96)72006-2
N.C. Gerber, S.G. Sligar, A role for Asp-251 in cytochrome P-450cam oxygen activation. Journal of Biological Chemistry. ,vol. 269, pp. 4260- 4266 ,(1994) , 10.1016/S0021-9258(17)41772-8
A L Shen, T D Porter, T E Wilson, C B Kasper, Structural analysis of the FMN binding domain of NADPH-cytochrome P-450 oxidoreductase by site-directed mutagenesis Journal of Biological Chemistry. ,vol. 264, pp. 7584- 7589 ,(1989) , 10.1016/S0021-9258(18)83274-4