Bovine thymus poly(adenosine diphosphate ribose) polymerase.
作者： Y. Tanaka , H. Ohgushi , H. Yoshihara , T. Kamiya , K. Yoshihara
DOI: 10.1016/S0021-9258(19)46955-X
关键词: Histone 、 Biochemistry 、 Polymerase 、 Base pair 、 Biology 、 Enzyme 、 Molecular biology 、 Enzyme assay 、 NAD+ kinase 、 DNA 、 Adenosine diphosphate ribose
摘要: Abstract About 1,300-fold purification of poly(adenosine diphosphate ribose) polymerase has been achieved from the extract bovine thymus with a recovery 10 to 20%. The final preparation purity 99%, and enzyme is composed single peptide molecular weight 130,000. purified required NAD+, Mg2+, thiol compound, DNA, histones for full activity. Whereas DNA essential activation enzyme, are not. observed stimulation reaction by shown be due masking inhibitory effect contaminating denartured in native preparation. concentration half-maximal activity (apparent Km DNA) proportional mixture. minimum estimation number nucleotide pairs one molecule 220 240 bulk calf while value fraction, "active DNA," which was separated fraction stage purification. These results suggest that activated binding specific site on DNA. apparent NAD+ maximum velocity estimated 60 micrometer 0.91 mumolper min per mg, respectively.
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