Comparison of various repetitive DNA elements as genetic markers for strain differentiation and epidemiology of Mycobacterium tuberculosis.

摘要: Five different genetic elements have been found to be associated with rearrangements in Mycobacterium tuberculosis complex strains. Of these elements, the insertion sequence IS6110 is presently most frequently used marker for strain differentiation of M. tuberculosis. In present study we compared five their potentials differentiate a given cluster Because presence only single copy or two copies at same chromosomal locus, large number strains could not differentiated by fingerprinting. Most strains, including low-copy-number fingerprinting 36-bp direct repeat polymorphic GC-rich repetitive DNA element. Less discriminative power was obtained major tandem and element IS1081. One which did contain encountered. Until now, this has invariantly all On basis classical taxonomic criteria sequencing 16S rRNA gene, shown genuine strain. Therefore, use as target polymerase chain reaction-facilitated detection should reconsidered.