In-Vitro Metabolites of Coumestrol

摘要: Coumestrol (COUM) is a phytoestrogen found in many plants. However, very little known about its metabolism and toxicity. We now report that COUM substrate for the cytochrome P-450-dependent monooxigenase (?) system. When was incubated with liver microsomes from differently pretreated rats, at least five metabolites were formed. The major metabolite (1) has been characterized by GC/MS. mass spectrum of trimethylsilyl derivative indicated monohydroxylated COUM. Incubation 1 catechol-O-methyltransferase/S-adenosyl-L-methionine, followed GC/MS analysis showed had catechol structure. effects different pretreatments on pattern oxidative studied. 3-Methylcholanthrene (MC)-induced yielded three times more than control microsomes, whereas no significant differences noted among phenobarbital-, isosafrol-, non-induced microsomes. This suggests P-450 isoenzymes inducible MC, probably 1A1 and/or 2A1, are involved formation 1.