The effects of high temperature level on square Bt protein concentration of Bt cotton

作者: Jun WANG , Yuan CHEN , Meng-hao YAO , Yuan LI , Yu-jin WEN

DOI: 10.1016/S2095-3119(15)61049-8

关键词: CultivarAnimal scienceNitrogen cycleSquare (algebra)BotanyAmino acidChemistryProteaseTransaminaseEnzymeBt cotton

摘要: Abstract Higher boll worm survival rates were detected after high temperature presented during square period in Bt cotton. The objective of this study was to investigate the effects level on efficacy two different types cotton cultivars at squaring stage. During 2011 2013 growth seasons, treatments ranged from 34 44°C climate chambers, and field experiments under weather with various levels conducted protein concentration nitrogen metabolism. chamber showed that insecticidal contents reduced 24 h elevated for both cultivars, whereas significant declines >38°C, only slightly numerical reductions observed when below 38°C. Similar responses also experimental sites 2013. Correspondingly, 38°C seems have little effect amino acid concentrations, soluble contents, glutamic-pyruvic transaminase (GPT) glutamic-oxalacetic (GOT) activities as well protease peptidase activities; however, above 38°C, enhanced decreased GPT GOT activities, bolstered detected. In general, higher is (>38°C), larger changes compound key enzymes cycle. findings indicated unstable insect resistance related

参考文章(23)
W.W. Umbreit, N.E. Tonhazy, N.G. White, A rapid method for the estimation of the glutamic-aspartic transaminase in tissues and its application to radiation sickness. Archives of Biochemistry and Biophysics. ,vol. 28, pp. 36- 42 ,(1950)
H. L. Halemani, S. S. Hallikeri, B. C. Patil, I. S. Katageri, V. C. Patil, Y. B. Palled, Influence of sowing time and moisture regimes on cry protein concentration and related parameters of Bt-cotton. Karnataka Journal of Agricultural Sciences. ,vol. 22, pp. 995- 1000 ,(2009)
J. Kaiser, Pests Overwhelm Bt Cotton Crop Science. ,vol. 273, pp. 423- ,(1996) , 10.1126/SCIENCE.273.5274.423
D. Richter, S. R. Penn, J. Greenplate, P. Dugger, M. Oppenhuizen, J. W. Mullins, Seasonal Cry1Ac levels in DP50B: the "Bollgard® basis" for Bollgard II. 2000 Proceedings Beltwide Cotton Conferences, San Antonio, USA, 4-8 January, 2000: Volume 2.. pp. 1039- 1040 ,(2000)
Xiang Zhang, Li Zhang, Guoyou Ye, Yonghui Wang, Yuan Chen, Dehua Chen, The impact of introducing the Bacillus thuringiensis gene into cotton on boll nitrogen metabolism Environmental and Experimental Botany. ,vol. 61, pp. 175- 180 ,(2007) , 10.1016/J.ENVEXPBOT.2007.05.008
E. W. Yemm, E. C. Cocking, R. E. Ricketts, The determination of amino-acids with ninhydrin Analyst. ,vol. 80, pp. 209- 214 ,(1955) , 10.1039/AN9558000209
E. S. Sachs, J. H. Benedict, D. M. Stelly, J. F. Taylor, D. W. Altman, S. A. Berberich, S. K. Davis, Expression and Segregation of Genes Encoding CryIA Insecticidal Proteins in Cotton Crop Science. ,vol. 38, pp. 1- 11 ,(1998) , 10.2135/CROPSCI1998.0011183X003800010001X
JiKun Huang, JianWei Mi, Hai Lin, ZiJun Wang, RuiJian Chen, RuiFa Hu, Scott Rozelle, Carl Pray, None, A decade of Bt cotton in Chinese fields: Assessing the direct effects and indirect externalities of Bt cotton adoption in China Science China Life Sciences. ,vol. 53, pp. 981- 991 ,(2010) , 10.1007/S11427-010-4036-Y
Yuan CHEN, Yu-jin WEN, Yuan CHEN, John Tom Cothren, Xiang ZHANG, Yong-hui WANG, William A Payne, De-hua CHEN, Effects of Extreme Air Temperature and Humidity on the Insecticidal Expression Level of Bt Cotton Journal of Integrative Agriculture. ,vol. 11, pp. 1836- 1844 ,(2012) , 10.1016/S2095-3119(12)60188-9
Carroll P. Vance, Gary H. Heichel, Donald K. Barnes, Jeff W. Bryan, Lois E. Johnson, Nitrogen Fixation, Nodule Development, and Vegetative Regrowth of Alfalfa (Medicago sativa L.) following Harvest Plant Physiology. ,vol. 64, pp. 1- 8 ,(1979) , 10.1104/PP.64.1.1