The impact of introducing the Bacillus thuringiensis gene into cotton on boll nitrogen metabolism

摘要: Abstract Changes in vegetative and reproductive growth have been widely observed Bacillus thuringiensis (Bt) transgenic cotton. The objective of this study was to investigate the characteristics boll nitrogen metabolism Bt cotton cultivars gain a better physiological understanding phenomenon. undertaken on two their parents, one conventional (GK22) its recurrent parent (Simian no. 3), other hybrid (Sikang 3) female 4), during 2003 2004 growing seasons at Yangzhou University Farm, Yangzhou, China. In study, total nitrogen, free amino acid soluble protein contents were investigated from flowering 50 days after (DAF). 2004, same parents measured for enzyme activities GPT, GOT Protease, which are key enzymes involved metabolism. results showed that developing period, cultivar lower than acid, protein, GPT GOT, but higher protease activity. totally opposite (GK22). biggest difference 14.8% decrease GK22 DAF, while Sikang 3, it an 8.1% increase 30 DAF. Similar obtained content, activities. However, changes activity direction, with being 30.6% 3 more 18.0% 4) before 40 Moreover, content reduced sharply. suggested had intensity development size weight. Therefore, cultural practices should aim increasing metabolic strength keep balance carbohydrate