Identification of membrane-associated proteins in Trypanosoma brucei encoding an internal, EARLRAEE amino acid repeat.

摘要: Differential screening of expression libraries with nonimmune and day 4-6 immune serum from naive rats infected the protozoan Trypanosoma lewisi was used to identify potential cell surface protein coding genes. Several T. cDNAs that resulted were partially characterized clone homologues brucei. The nucleotide sequence encoding Tb-29 genes parasitic brucei obtained by this method (3557 nucleotides for Tb-291 8729 Tb-292) encoded predicted open reading frames 1070 2550 amino acids, respectively. proteins a large domain an octapeptide (EARLRAEE) repeat (79 repeats in 60 Tb-292), which shared significant similarity S-antigen isolate NF7 Plasmodium falciparum. acid Tb-292 transmembrane domains (eight total). Indirect immunofluorescence using confocal image analysis immunoelectron microscopy located EARLRAEE membranous network. most abundantly distributed area surrounding nucleus, region between nucleus flagellar pocket, immediately underneath pocket membrane. subcellular distribution suggests these may provide constituent associated cell's vesicular transport system.